Yingang Feng, Ph. D.
Professor

E-mail: fengyg@qibebt.ac.cn

Research Interests
--Structure, function, and engineering of proteins from energy microorganisms and plants
--Structure and interactions of cancer-related proteins
--Archaeal proteins
--Biomolecular NMR/Protein structure, interaction and function/Protein-nucleic acid interaction
--Metabolomics and metabolic engineering of energy microorganisms

Education and Academic Experience
1995 Sep. - 1999 Jun.     Bachelor, Department of Physics, Nanjing University
1999 Sep. – 2004 Jun.    Ph.D. (Biochemistry and Molecular Biology), Institute of Biophysics, Chinese Academy of Sciences
2004 Jul. – 2006 Jun.      Postdoctoral Researcher, Beijing NMR Center, College of Chemistry and Molecular Engineering, Peking University
2006 Jul. – 2011 May    Associate Professor, Institute of Biophysics, Chinese Academy of Sciences
2011 Jun. – now              Professor, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

Funds

  1. National Natural Science Foundation of China, 2013-2016, Structure, interaction and rational design of the cellulosome-assembling modules from Clostridium studied by NMR spectroscopy
  2. The National High Technology Research and Development Program of China (863 project), 2012-2015, Construction and application of artificial cell factory utilizing light energy.
  3. National Natural Science Foundation of China, 2012-2015, Structure and interactions of drug target NrdH-redoxin from Mycobacterium tuberculosis by NMR spectroscopy
  4. National Natural Science Foundation of China, 2010-2012, Structure and interactions of tumor suppression protein RBBP1 by NMR spectroscopy
  5. National Natural Science Foundation of China, 2009-2011, The interactions between translationally controlled tumor protein and signaling proteins studied by nuclear magnetic resonance
  6. Beijing Natural Science Foundation, 2009-2011, The interactions between translationally controlled tumor protein and other signaling proteins
  7. China Postdoctoral Science Foundation, 2004-2006, Structure and Function of Poplar Glutaredoxin Grx1 by NMR Spectroscopy

Publications [ResearcherID] [PubMed]

Research Papers

  1. Xi Liu, Shian Liu, Yingang Feng*, Jian-Zhong Liu, Yuling Chen, Khanh Pham, Haiteng Deng, Kendal D. Hirschi, Xinquan Wang*, and Ninghui Cheng* (2013) Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts. Proc. Natl. Acad. Sci. USA 110, in press. (*Corresponding author)
    http://dx.doi.org/10.1073/pnas.1306899110
  2. Tao Xie†, Yingang Feng†, Lu Shan, and Jinfeng Wang* (2013) Modeling of the [E43S]SNase-ssDNA-Cd2+ complex: structural insight into the action of nuclease on ssDNA. Arch. Biochem. Biophys. 532(2),103-113. (†Co-first author)
    http://dx.doi.org/10.1016/j.abb.2013.02.003
  3. Mang Gao, Xiaojin Song*, Yingang Feng, Wenli Li, and Qiu Cui (2013) Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116. J. Oleo Sci. 62(3), 143-151.
    http://dx.doi.org/10.5650/jos.62.143
  4. Yan Xiao, Jingtao Zhang, Jiatao Cui, Yingang Feng, Qiu Cui* (2013) Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress. Bioresour. Technol.130, 731-738.
    http://dx.doi.org/10.1016/j.biortech.2012.11.116
  5. Zhenling Cui, Yifei Li*, Yan Xiao, Yingang Feng, and Qiu Cui* (2013) Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824. Biomol. NMR Assign.7(1), 73-76.
    http://dx.doi.org/10.1007/s12104-012-9381-2
    Erratum: Biomol. NMR Assign. 7(1), 77.
    http://dx.doi.org/10.1007/s12104-012-9386-x
  6. Ting Song, Xiangqian Li, Xilong Chang, Xiaomeng Liang, Yan Zhao, Guiye Wu, Shenghui Xie, Pengchen Su, Zhiyong Wu, Yingang Feng, Zhichao Zhang* (2013) 3-Thiomorpholin-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile (S1) derivatives as pan-Bcl-2-inhibitors of Bcl-2, Bcl-xL and Mcl-1. Bioorg. Med. Chem. 21(1), 11-20. http://dx.doi.org/10.1016/j.bmc.2012.11.008
  7. Zhichao Zhang*, Ting Song, Xiangqian Li, Zhiyong Wu, Yingang Feng, Feibo Xie, Chengwu Liu, Jianquan Qin, Hongbo Chen* (2013) Novel soluble myeloid cell leukemia sequence 1 (Mcl-1) inhibitor (E,E)-2-(benzylaminocarbonyl)-3-styrylacrylonitrile (4g) developed using a fragment-based approach. Eur. J. Med. Chem. 59(1), 141-149.
    http://dx.doi.org/10.1016/j.ejmech.2012.10.050
  8. Zhichao Zhang*, Xiangqian Li, Ting Song, Yan Zhao, and Yingang Feng (2012) An anthraquinone scaffold for putative, two-face Bim BH3 α-helix mimicries. J. Med. Chem.55(23), 10735–10741.
    http://dx.doi.org/10.1021/jm301504b
  9. Shan Wang, Gu-Zhen Cui, Xiang-Fei Song, Yingang Feng* and Qiu Cui* (2012) Efficiency and stability enhancement of cis-epoxysuccinic acid hydrolase by fusion with a carbohydrate binding module and immobilization onto cellulose. Appl. Biochem. Biotechnol. 168(3), 708-717. (*Corresponding author)
    http://dx.doi.org/10.1007/s12010-012-9811-8
  10. Sanduo Zheng†, Jia Wang†, Yingang Feng†*, Jinfeng Wang and Keqiong Ye* (2012) Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases. PLoS One 7(9), e45437. (†Co-first author; *Corresponding author)
    http://dx.doi.org/10.1371/journal.pone.0045437
  11. Lei Wang, Bingjie Ouyang, Yifei Li, Yingang Feng, Jean-Pierre Jacquot, Nicolas Rouhier and Bin Xia* (2012) Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin. Protein Cell 3(9), 714-721.
    http://dx.doi.org/10.1007/s13238-012-2051-4
  12. Hongwei Yao, Yingang Feng* and Jinfeng Wang (2012) Dimer form of archaeal protein SSO6904 identified by NMR. Chinese J. Magn. Reson. 29(2), 224-230. (In Chinese) (*Corresponding author)
    http://magres.wipm.ac.cn/CN/abstract/abstract9507.shtml
  13. Gu-Zhen Cui, Shan Wang, Yifei Li, Yi-Jun Tian, Yingang Feng, and Qiu Cui* (2012) High yield recombinant expression, characterization and homology modeling of two types of cis-epoxysuccinic acid hydrolases. Protein J. 31(5), 432-438.
    http://dx.doi.org/10.1007/s10930-012-9418-5
  14. Yingang Feng†, Yong Geng†, Tao Zhou, and Jinfeng Wang* (2012) NMR structure note: human esophageal cancer-related gene 2. J. Biomol. NMR 53(1), 65-70. (†Co-first author)
    http://dx.doi.org/10.1007/s10858-012-9622-9
  15. Gu-zhen Cui, Wei Hong, Jie Zhang, Wen-li Li, Yingang Feng, Ya-jun Liu*, and Qiu Cui* (2012) Targeted gene engineering in Clostridium cellulolyticum H10 without methylation. J. Microbiol. Methods 89(3), 201-208.
    http://dx.doi.org/10.1016/j.mimet.2012.02.015
  16. Hongwei Yao, Yingang Feng, Tao Zhou, Jinfeng Wang*, and Zhi-Xin Wang (2012) NMR studies of the interaction between human programmed cell death 5 and human p53. Biochemistry 51(13), 2684-2693.
    http://dx.doi.org/10.1021/bi201822x
  17. Weibin Gong, Tao Zhou, Jinjin Mo, Sarah Perrett, Jinfeng Wang* and Yingang Feng* (2012) Structural insight into recognition of methylated histone tails by retinoblastoma-binding protein 1. J. Biol. Chem. 287(11), 8531-8540. (*Corresponding author)
    http://dx.doi.org/10.1074/jbc.M111.299149
  18. Jinsong Xuan*, Xiaxia Song, Jinfeng Wang, Yingang Feng* (2011) Resonance assignments of a putative PilT N-terminus domain protein SSO1118 from hyperthermophilic archaeon Sulfolobus solfataricus P2. Biomol. NMR Assign. 5(2), 161-164. (*Corresponding author)
    http://dx.doi.org/10.1007/s12104-010-9291-0
  19. Yi-Tong Liu, Qiong-Jie Dan, Jiawei Wang, Yingang Feng, Lei Chen, Juan Liang, Qinxi Li, Sheng-Cai Lin, Zhi-Xin Wang, and Jia-Wei Wu* (2011) Molecular basis of Wnt activation via the DIX-domain protein Ccd1. J. Biol. Chem. 286(10), 8597-8608.
    http://dx.doi.org/10.1074/jbc.M110.186742
  20. Maxim S. Titushin†, Yingang Feng†, Galina A. Stepanyuk, Yang Li, Svetlana V. Markova, Stefan Golz, Bi-Cheng Wang, John Lee, Jinfeng Wang, Eugene S. Vysotski, and Zhi-Jie Liu* (2010) NMR derived topology of a GFP-photoprotein energy transfer complex. J. Biol. Chem. 285(52), 40891-40900. (†Co-first author)
    http://dx.doi.org/10.1074/jbc.M110.133843
  21. Tao Zhou, Jinzhong Lin, Yingang Feng, and Jinfeng Wang* (2010) Binding of NADPH destabilizes the iron-sulfur clusters of human mitoNEET. Biochemistry 49(44), 9604-9612.
    http://dx.doi.org/10.1021/bi101168c
  22. Yingang Feng, Hongwei Yao, and Jinfeng Wang* (2010) Crystal Structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex. Protein Sci. 19(6), 1253-1257.
    http://dx.doi.org/10.1002/pro.385
  23. Min Wang, Yingang Feng, Hongwei Yao and Jinfeng Wang* (2010) Importance of the C-terminal loop L137-S141 for the folding and folding stability of staphylococcal nuclease. Biochemistry 49(20), 4318-4326
    http://dx.doi.org/10.1021/bi100118k
  24. Yingang Feng*, Hongwei Yao, and Jinfeng Wang* (2010) Solution structure and calcium binding of protein SSO6904 from the hyperthermophilic archaeon Sulfolobus solfataricus. Proteins 78(2), 474-479 (*Corresponding author)
    http://dx.doi.org/10.1002/prot.22580
  25. Yong Geng†, Yingang Feng†, Tao Xie, Lu Shan and Jinfeng Wang* (2009) The native-like interactions between SNase121 and SNase(111-143) fragments induce the recovery of their native-like structures and the ability to degrade DNA. Biochemistry 48(36), 8692-8703. (†Co-first author)
    http://dx.doi.org/10.1021/bi901099s
  26. Hongwei Yao, Lanjun Xu, Yingang Feng, Dongsheng Liu, Yingyu Chen* and Jinfeng Wang* (2009) Structure-function correlation of human programmed cell death 5 protein. Arch. Biochem. Biophys.486(2), 141-149.
    http://dx.doi.org/10.1016/j.abb.2009.03.018
  27. Jinsong Xuan, Hongwei Yao, Yingang Feng and Jinfeng Wang* (2009) Cloning, expression and purification of DNA binding protein Mvo10b from Methanococcus voltae. Protein Expr. Purif. 64(2), 162-166.
    http://dx.doi.org/10.1016/j.pep.2008.11.003
  28. Shilong Fan†, Yingang Feng†, Zhiyi Wei, Bin Xia* and Weimin Gong* (2009) Solution structure of synbindin atypical PDZ domain and interaction with syndecan-2. Protein Peptide Lett.16(2), 189-195. (†Co-first author)
    http://dx.doi.org/10.2174/092986609787316342
  29. Xianyang Fang, Yingang Feng and Jinfeng Wang* (2009) Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b. Arch. Biochem. Biophys. 481(1), 52-58.
    http://dx.doi.org/10.1016/j.abb.2008.08.025
  30. Xianyang Fang, Qiu Cui, Yufeng Tong, Yingang Feng, Lu Shan, Li Huang and Jinfeng Wang* (2008) A stabilizing alpha/beta-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry 47(43), 11212-11221.
    http://dx.doi.org/10.1021/bi8007593
  31. Yong Geng, Yingang Feng, Tao Xie, Yuanyuan Dai, Jinfeng Wang* and Shih-Hsin Lu (2008) Mapping the putative binding site for uPA protein in Esopageal Cancer Related Gene 2 by heteronuclear NMR method. Arch. Biochem. Biophys. 479(2), 153-157.
    http://dx.doi.org/10.1016/j.abb.2008.08.023
  32. Li Guo†, Yingang Feng†, Zhenfeng Zhang, Hongwei Yao, Yuanming Luo, Jinfeng Wang* and Li Huang* (2008) Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 36(4), 1129-1137. (†co-first author)
    http://dx.doi.org/10.1093/nar/gkm1128
  33. Yingang Feng†, Dongsheng Liu†, Hongwei Yao and Jinfeng Wang* (2007) Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR. Arch. Biochem. Biophys. 467(1), 48-57. (†co-first author)
    http://dx.doi.org/10.1016/j.abb.2007.08.021
  34. Yong Geng, Min Wang, Tao Xie, Yingang Feng and Jinfeng Wang* (2007) Folding of the C-terminal fragment V111-D143 of staphylococcal nuclease in aqueous solution. Protein Peptide Lett. 14(8), 747-755.
    http://dx.doi.org/10.2174/092986607781483769
  35. Tao Xie, Dongsheng Liu, Yingang Feng, Lu Shan and Jinfeng Wang*. (2007) Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease. Biophys. J. 92(6), 2090-2107.
    http://dx.doi.org/10.1529/biophysj.106.092155
  36. Yingang Feng, Nan Zhong, Nicolas Rouhier, Toshiharu Hase, Masami Kusunoki, Jean-Pierre Jacquot, Changwen Jin and Bin Xia* (2006) Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site. Biochemistry, 45(26), 7998-8008.
    http://dx.doi.org/10.1021/bi060444t
  37. Dongsheng Liu, Hongwei Yao, Yaoyao Chen, Yingang Feng, Yingyu Chen and Jinfeng Wang* (2005) The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential character. Biochem. J. 392(1), 47-54.
    http://dx.doi.org/10.1042/BJ20050688
  38. Yingang Feng, Nicolas Rouhier, Jean-Pierre Jacquot and Bin Xia* (2005) Letter to the Editor: 1H, 15N, and 13C Resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides. J. Biomol. NMR, 31(3), 263-264.
    http://dx.doi.org/10.1007/s10858-005-0497-x
  39. Jixun Dai, Xu Wang, Yingang Feng, Guibao Fan and Jinfeng Wang* (2004). Searching for folding initiation sites of staphylococcal nuclease: a study of N-terminal short fragments. Biopolymers, 75(3), 229-241.
    http://dx.doi.org/10.1002/bip.20121
  40. Dongsheng Liu, Yingang Feng, Yuan Cheng and Jinfeng Wang* (2004) Human programmed cell death 5 protein has a helical-core and two dissociated structural regions. Biochem. Biophys. Res. Commun.318(2), 391-396.
    http://dx.doi.org/10.1016/j.bbrc.2004.04.044
  41. Qiu Cui, Yufeng Tong, Hong Xue, Li Huang, Yingang Feng and Jinfeng Wang* (2003) Two conformations of archaeal Ssh10b: the origin of its temperature-dependent interaction with DNA. J. Biol. Chem. 278(51), 51015-51022.
    http://dx.doi.org/10.1074/jbc.M308510200
  42. Yingang Feng, Dongsheng Liu and Jinfeng Wang* (2003) Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy. J. Mol. Biol. 330(4), 821-837.
    http://dx.doi.org/10.1016/S0022-2836(03)00660-0
  43. Yufeng Tong, Qiu Cui, Yingang Feng, Li Huang and Jinfeng Wang* (2002) Letters to the Editor: 1H, 15N, and 13C resonance assignments and secondary structure of the Ssh10b from hyperthermophilic archaeon Sulfolobus shibatae. J. Biomol. NMR, 22(4), 385-386.
    http://dx.doi.org/10.1023/A:1014932202874

Reviews

  1. Jinsong Xuan* and Yingang Feng* (2012) The archaeal Sac10b protein family: conserved proteins with divergent functions. Curr. Protein Pept. Sci. 13(3), 258-266. (Review) (*Corresponding author)
    http://dx.doi.org/10.2174/138920312800785067
  2. Maxim S. Titushin, Yingang Feng, John Lee, Eugene S. Vysotski, Zhi-Jie Liu* (2011) Protein-protein complexation in bioluminescence. Protein Cell 2(12), 957-972. (Review; Cover paper)
    http://dx.doi.org/10.1007/s13238-011-1118-y
  3. Min Li*, Yingang Feng, Yang Gao and Qingyu Wu (2007) Glutaredoxin system and its regulation to the cytosolic thiol-redox status. Acta Biophys. Sin. 23(5), 343-350. (Review in Chinese)
    http://www.cjb.org.cn/CN/abstract/abstract14752.shtml#

Others

  1. Yingang Feng (2009) Cover story: identification and solution structure of Cren7, a crenarchaea conserved chromatin protein. Acta Biophys. Sin. 2009, 25(3), cover. (In Chinese)
    http://www.cjb.org.cn/CN/volumn/volumn_390.shtml
  2. Joint author (2008) A review of the joint 52nd Annual Meeting of the Biophysical Society and 16th IUPAB International Biophysics Congress. Acta Biophys. Sin. 24(2), 161-166 (In Chinese)
    http://www.cjb.org.cn/CN/abstract/abstract14467.shtml

Meetings
--The 17th National Magnetic Resonance Conference, Xiamen (2012) Oral report
--The 16th National Magnetic Resonance Conference, Haikou (2010) Oral report
--Joint Conference of the Asian Crystallographic Association and Chinese Crystallographic Society, Beijing (2009) Poster
--2009 Symposium of Nuclear Magnetic Resonance for Biological Macromolecules, Hefei (2009)
--2009 Annual Symposium on Chinese Frontiers of Science & Pre-meeting of the 12th Annual Symposium on Chinese-American Frontiers of Science, Beijing (2009)
--The joint 52nd Annual Meeting of the Biophysical Society and 16th IUPAB International Biophysics Congress, Long Beach, California, USA (2008) Poster
--The 15th National Magnetic Resonance Conference, Ningbo (2008) Oral report
--The 2nd Cross-Strait Magnetic Resonance Symposium, Hangzhou (2008) Oral report
--Bruker Annual NMR Technique Conference, Yichang (2008) Oral report
--Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing (2007) Oral report
--The 4th International Conference on Structural Genomics, Beijing (2006) Poster
--Bruker Annual NMR Technique Conference, Haikou (2005) Oral report
--Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing (2005) Poster
--Bruker Annual NMR Technique Conference, Kunming (2003) Oral report
--The 12th National Magnetic Resonance Conference, Dalian (2002) Poster